Immunofluorescence:Article Title:
Article Snippet: .. Antibodies used for immunofluorescence and western blotting Rabbit anti-YTHDC2 (1:500 or 1:2,000 when TSA used, A303-026A, (RRID:AB_10754785), Bethyl laboratories), mouse anti-SYCP3 (1:200, clone [Cor 10G11/7], (RRID:AB_10678841), Abcam), rabbit anti-SYCP3 (1:500, NB300-232, (RRID:AB_2087193), Novus Biologicals), rabbit anti-SYCP1 (1:500, NB300-229SS, (RRID:AB_922549), Novus Biologicals), rabbit anti-HORMAD1 (1:1,000, 13917-1-AP, (RRID:AB_2120844), Proteintech), guinea pig anti-H1t (1:500, Mary Ann Handel (1)), human anti-centromere (1:50, 15-234, (RRID:AB_2939058), Antibodies Incorporated), rabbit anti-RAD51 (1:200 [N1C2], (RRID:AB_1951602), GeneTex), goat anti-VASA (1:200, AF2030, (RRID:AB_2277369), R&D Systems), mouse anti-phospho-Histone H2A.X (Ser139) (1:500 clone [JBW301], (RRID:AB_309864), Millipore), rabbit anti-CYCLIN A2 (1:250 clone [EPR17351], (RRID:AB_2890136), Abcam), rabbit anti-MEIOC (1:1,000 IF, David Page (2)), mouse anti-DCP1A (1:250 clone [3G4], (RRID:AB_1843673), Sigma), goat anti-PIWIL1 (1:200, AF6548, (RRID:AB_10971944), Novus Biologicals). .. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining was done following manufacturer’s instruction (In Situ cell Death Detection Kit TMR red, Roche).
Western Blot:Article Title:
Article Snippet: .. Antibodies used for immunofluorescence and western blotting Rabbit anti-YTHDC2 (1:500 or 1:2,000 when TSA used, A303-026A, (RRID:AB_10754785), Bethyl laboratories), mouse anti-SYCP3 (1:200, clone [Cor 10G11/7], (RRID:AB_10678841), Abcam), rabbit anti-SYCP3 (1:500, NB300-232, (RRID:AB_2087193), Novus Biologicals), rabbit anti-SYCP1 (1:500, NB300-229SS, (RRID:AB_922549), Novus Biologicals), rabbit anti-HORMAD1 (1:1,000, 13917-1-AP, (RRID:AB_2120844), Proteintech), guinea pig anti-H1t (1:500, Mary Ann Handel (1)), human anti-centromere (1:50, 15-234, (RRID:AB_2939058), Antibodies Incorporated), rabbit anti-RAD51 (1:200 [N1C2], (RRID:AB_1951602), GeneTex), goat anti-VASA (1:200, AF2030, (RRID:AB_2277369), R&D Systems), mouse anti-phospho-Histone H2A.X (Ser139) (1:500 clone [JBW301], (RRID:AB_309864), Millipore), rabbit anti-CYCLIN A2 (1:250 clone [EPR17351], (RRID:AB_2890136), Abcam), rabbit anti-MEIOC (1:1,000 IF, David Page (2)), mouse anti-DCP1A (1:250 clone [3G4], (RRID:AB_1843673), Sigma), goat anti-PIWIL1 (1:200, AF6548, (RRID:AB_10971944), Novus Biologicals). .. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining was done following manufacturer’s instruction (In Situ cell Death Detection Kit TMR red, Roche).
|
Bethyl is a verified supplier 